Venn Diagram¶
The Venn tab shows the overlap of differentially expressed genes (DEGs) across 2–3 contrasts. This helps identify genes that are consistently regulated across experimental conditions or unique to specific comparisons.
Requirements¶
Your project must have at least 2 contrast files (*.deseq2.results_filtered.tsv) to use this tab.
Controls¶
| Control | Description |
|---|---|
| Select contrasts | Choose 2 or 3 contrasts to compare (multiselect, max 3) |
| DEG direction | All DEGs — shows 3 side-by-side Venns (Up, Down, All); Up-regulated or Down-regulated — shows a single Venn |
Reading the Diagram¶
- Each circle represents one contrast, labeled with the contrast name and total DEG count. Circle colors are derived from the sidebar Down, Up, and Select color settings.
- Overlapping regions show genes significant in multiple contrasts
- Numbers in each region indicate gene counts
- Font color and size are controlled by the sidebar Appearance settings. Region counts use a larger size relative to the base font; contrast labels use a slightly smaller size.
- Long contrast names automatically wrap across multiple lines so they don't overflow the diagram
When "All DEGs" is selected, three Venn diagrams appear side by side:
- Up-regulated — genes with positive log2FC above threshold
- Down-regulated — genes with negative log2FC below threshold
- All DEGs — combined up and down
Summary Metrics¶
A row of metric cards shows per-contrast totals: total DEGs, up count, and down count.
Gene Lists¶
Below the diagram, expandable sections list genes in each Venn region:
- Gene ID, log2FC, and adjusted p-value from each contrast
- Download button to export each region's gene list as CSV
Tip
Use the Venn diagram to find shared biomarkers — genes that are significant across multiple contrasts are often more robust candidates.