GO — Gene Ontology Enrichment¶
Header: GO: Gene Ontology Enrichment
The GO tab presents Gene Ontology enrichment analysis results, showing which biological functions, cellular locations, or molecular activities are over-represented among your differentially expressed genes.
GO-Specific Controls¶
The GO tab has controls that appear above the chart, in addition to the global sidebar filters. The contrast is determined by the Contrast dropdown in the sidebar — switching the sidebar contrast automatically updates the GO report.
Direction¶
Dropdown to filter by expression direction:
- up — Show GO terms enriched among upregulated genes.
- down — Show GO terms enriched among downregulated genes.
- all — Show GO terms enriched across all differentially expressed genes.
Ontology¶
Dropdown to select which Gene Ontology domain to display:
- BP — Biological Process (what biological activities the genes participate in)
- CC — Cellular Component (where in the cell the gene products are located)
- MF — Molecular Function (what molecular activities the gene products perform)
Top N terms¶
Controlled by the Top N slider in the sidebar (range: 5–50, default: 20). Determines how many GO terms are displayed in the chart.
GO Enrichment Bar Chart¶
The main visualization is a horizontal bar chart where each bar represents a GO term. The x-axis shows Gene Count (number of differentially expressed genes annotated with that term), and bars are colored by -log10(p.adjust) — a color scale indicating statistical significance, with more saturated colors representing more significant enrichment.
The chart title dynamically reflects your current selections (e.g., "GO Enrichment — skin | up | BP (Top 15)").
GO Enrichment Table (Expandable)¶
Below the chart, an expandable GO Enrichment Table provides the full tabular data behind the chart, including GO term IDs, descriptions, gene counts, p-values, and adjusted p-values.