Enrichment Compare — Cross-Contrast Enrichment Heatmap¶
Header: Enrichment Compare: Cross-Contrast Enrichment Heatmap
The Enrichment Compare tab displays a heatmap comparing GO or KEGG enrichment significance across multiple contrasts. This makes it easy to spot which biological pathways or terms are consistently enriched or unique to specific comparisons.
Requirements¶
Your project must have at least 2 contrasts with enrichment data (GO or KEGG) to use this tab.
Controls¶
| Control | Description |
|---|---|
| Database | Select GO or KEGG enrichment data |
| Direction | Filter by regulation direction: up, down, or all |
| Top terms per contrast | Number of top significant terms to include per contrast (5–30, default: 10) |
| Ontology | (GO only) Filter by ontology category: Biological Process (BP), Cellular Component (CC), or Molecular Function (MF) |
The adjusted p-value threshold from the sidebar Filters section is used to determine significance.
Heatmap¶
The heatmap displays -log10(adjusted p-value) for each term across contrasts:
- Rows = enrichment terms (sorted by mean significance)
- Columns = contrasts
- Color intensity = higher values (more significant) are more intense
- Color scheme adapts to direction: Up uses the sidebar "Up" color, Down uses the sidebar "Down" color, and "all" uses a yellow-to-red scale
- Height scales automatically based on the number of terms, respecting the sidebar Plot Height as a minimum
Comparison Matrix (Expandable)¶
Below the heatmap, an expandable table shows the raw -log10(p.adjust) values for all terms across contrasts. Download as CSV for further analysis.
Tip
Compare the same ontology category (e.g., Biological Process) across contrasts to identify shared and unique biological themes in your experimental conditions.